Detection of human papillomavirus DNA in genital condylomata in women and their male partners by using in situ hybridization with digoxygenin labeled probes.
نویسندگان
چکیده
منابع مشابه
Human papillomavirus DNA detection in male sexual partners of women with genital human papillomavirus infection.
OBJECTIVES To determine the prevalence of human papillomavirus (HPV) DNA in the male partners of HPV-infected women, assess the concordance of the viral group in the infected pair, define the most affected sites in the male genitalia, and compare diagnostic methods in men. METHODS Fifty male, stable sexual partners of women positive for HPV DNA by the Hybrid Capture 2 (hc2) test had material ...
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A modified, commercially available DNA-DNA in situ hybridization test that uses biotinylated probes for the identification of human papillomavirus (HPV) DNA types 6/11, 16/18, and 31/33/35 was evaluated. HPV DNA was detected in 314 of 787 (40%) histologically abnormal genital biopsy specimens by using the ViraType in situ assay (Life Technologies, Gaithersburg, Md.), in which the hybridization ...
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Detection of human papillomavirus DNA in anogenital condylomata in men using in situ DNA hybridisation applied to paraffin sections.
An in situ DNA hybridisation method was used to detect human papillomavirus (HPV) DNA (HPV types 6, 11, 16, and 18), and an immunoperoxidase (IP-PAP) method to detect HPV structural protein expression in paraffin sections of biopsy specimens from 133 men treated for penile (in 114 cases) and anal (in 19 cases) warts. The anatomical distribution on the penis of classic condyloma acuminatum and o...
متن کاملSensitive two-color whole-mount in situ hybridizations using digoxygenin- and dinitrophenol-labeled RNA probes.
Two-color whole-mount in situ hybridization is a powerful technique for comparing the spatial expression patterns of two genes in developing embryos or within a given adult tissue. Many current procedures use two genespecific antisense RNA probes, each incorporating a different chemical moiety that is recognized by a specific antibody. Typically, the first gene-specific RNA probe is labeled wit...
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ژورنال
عنوان ژورنال: The Tohoku Journal of Experimental Medicine
سال: 1990
ISSN: 0040-8727,1349-3329
DOI: 10.1620/tjem.160.383